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Conventional Microarray Technology
Glass
Substrate
DNA “probe”
DNA “target”
•Up to tens of thousands of DNA probes are covalently attached to
glass by epoxysilane
coupling agent
•mRNA
isolated from cell cultures is converted into fluorescently-labeled DNA
•Homologous,
labeled DNA targets bind DNA probes
•Non-homologous targets are prevented from binding by 62°C
hybridization temperature
and subsequent washes•Fluorescent
excitation is used to assess binding events
Fluorescent emission
A.
B.
Glass
Substrate