•Target DNA binds
non-specifically and yields background noise
•
•Labeling procedure is inherently
inefficient and 90% of target is lost in process
•
•Uniform hybridization
temperature used to decouple non-homologous targets from probes fails
to account for variations in target-to-probe binding energies
•
•Insensitive to subtle
changes in gene expression (< 1.5-fold)
•
•Requires large, expensive
equipment and is arduous
•
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